CD33 Rabbit Monoclonal Antibody [Clone ID: M195]

Specifications

Product Data
Clone Name	M195
Applications	FC, IF, R
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing mice with live leukemic myeloblasts.
Specificity	This antibody binds CD33, which is a 67 kD glycoprotein expressed on monocytes, hematopoetic progenitors, early myeloid progenitor cells and myeloid leukemias (acute and chronic) but not expressed on mature myeloid cells, normal stem cells, other hemtopoietic cells and non hematopoietic human tissues. This antibody is recommended for myeloid differentiation studies, diagnosis of acute non-lymphoid leukemia cells and therapeutic treatment of myelois leukemia. Specificity of this antibody to CD33 was determined using RIA and immunoabsorption. Immunoflurorescence assay showed that the antigen is not expressed on adult human tissues (PMID: 2716349). Flow cytometric analysis was used to confirm reactivity of M195 with fresh hematopoietic neoplasms in the blood or bone marrow. In combination with MY9, this antibody is useful in diagnosis of acute myelogenous leukemia(AML) (PMID: 2725060). Murine antibody M195 was used in the phase I trial of AML (PMID: 1999719). Humanized IgG1 and IgG3 versions of this antibody has higher apparent binding affinity for CD33 antigen than the chimeric and parent murine version (PMID: 1737932, 1458463). The increase in binding affinity resulted from eliminating an N-linked glycosylation site at residue 73 in the heavy chain variable region in the course of humanization (PMID: 8232322). Preliminary trials to treat AML with humanized antibody showed specific bone marrow targeting without an immunogenic response (PMID: 8124221).
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	CD33 molecule
Database Link	Entrez Gene 945 Human UniProt ID P20138
Synonyms	FLJ00391; gp67; p67; SIGLEC-3; SIGLEC3
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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