CD40 Rabbit Monoclonal Antibody [Clone ID: A54 scFv]

Specifications

Product Data
Clone Name	A54 scFv
Applications	Bl, ELISA
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	A54 human anti-CD40 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcγ fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.
Specificity	A54 binds specifically to the D1 domain of CD40 (and can only bind to the intact i.e. non-truncated version of CD40 and when an AD2 peptide tag has not been added) and can recognise CD40 on a B cell line (BJAB). This scFv binds to CD40 (immobilized by amine coupling) with a KD within the range of 40nM. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells and some epithelium including the thymus. CD40 binds to the membrane protein CD154 of the TNF superfamily and this interaction is required for the seconfary immune responses and germinal centre formation. A54 is able to block the CD40-CD154 interaction by around 60-80% and can also induce CD40 sinalling in human B cells when at 'unusually high concentrations' (The CD40 receptor - target, tool and technology, Ellmark, 2002). This scFv can also be used for ELISA studies (PMID: 12153507)
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	CD40 molecule
Database Link	Entrez Gene 958 Human UniProt ID P25942
Synonyms	Bp50; CDW40; MGC9013; p50; TNFRSF5
Note	This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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