Trp53 Rabbit Monoclonal Antibody [Clone ID: R01-2B9]

Specifications

Product Data
Clone Name	R01-2B9
Applications	IF, IP, WB
Recommend Dilution	WB: 1/1000 ICC/IF: 1/20 IP: 1/20
Reactivity	Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	Recombinant protein of mouse p53
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 44 kDa; Observed MW: 53 kDa
Gene Name	transformation related protein 53
Database Link	Entrez Gene 22059 Mouse UniProt ID P02340
Background	Swiss-Prot Acc.P02340.Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression . Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Synonyms	bbl; bfy; bhy; p44; p53; Tp53
Reference Data

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