SF2 (SRSF1) Rabbit Monoclonal Antibody [Clone ID: R07-9G7]

Specifications

Product Data
Clone Name	R07-9G7
Applications	IF, IHC, WB
Recommend Dilution	WB: 1/2000 IHC: 1/50 ICC/IF: 1/20-1/100
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human SF2
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 28 kDa; Observed MW: 28 kDa
Gene Name	serine/arginine-rich splicing factor 1
Database Link	Entrez Gene 6426 Human UniProt ID Q07955
Background	Swiss-Prot Acc.Q07955.Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
Synonyms	1110054N12Rik; 5730507C05Rik; 6330415C05Rik; AI482334; Asf; AW491331; Sf2; Sfrs1
Reference Data

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