SMAD2 Mouse Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1/1000
Reactivity	Human, Rat, Mouse
Host	Mouse
Clonality	Polyclonal
Immunogen	Recombinant Protein of Smad3
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Observed MW(kDa):52/60
Gene Name	SMAD family member 2
Database Link	Entrez Gene 4087 Human UniProt ID P84022
Background	Swiss-Prot Acc.P84022.Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 . Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses .
Synonyms	hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; MGC22139; MGC34440
Reference Data

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