SET Rabbit Monoclonal Antibody [Clone ID: R04-5E8]

Specifications

Product Data
Clone Name	R04-5E8
Applications	IF, IHC, IP, WB
Recommend Dilution	WB: 1/2000-1/10000 IHC: 1/50 ICC/IF: 1/200 IP: 1/20
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human SET
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 34 kDa; Observed MW: 39 kDa
Gene Name	SET nuclear proto-oncogene
Database Link	Entrez Gene 6418 Human UniProt ID Q01105
Background	Swiss-Prot Acc.Q01105.Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Synonyms	2PP2A; I-2PP2A; I2PP2A; IGAAD; IPP2A2; PHAPII; TAF-I; TAF-IBETA
Reference Data

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