Securin (PTTG1) Rabbit Monoclonal Antibody [Clone ID: R09-6J7]

Specifications

Product Data
Clone Name	R09-6J7
Applications	IHC, IP, WB
Recommend Dilution	WB: 1/1000-1/5000 IHC: 1/20 IP: 1/20
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human Securin
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 22 kDa; Observed MW: 25 kDa
Gene Name	pituitary tumor-transforming 1
Database Link	Entrez Gene 9232 Human UniProt ID O95997
Background	Swiss-Prot Acc.O95997.Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
Synonyms	EAP1; HPTTG; MGC126883; MGC138276; OTTHUMP00000160845; PTTG; securin; TUTR1
Reference Data

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