PMS2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	WB: 1/500-1/2000 IHC: 1/50-1/200 ICC/IF: 1/50-1/200 IP: 1/50 FC: 1/50
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthesized peptide derived from human PMS2
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	110kDa
Gene Name	PMS1 homolog 2, mismatch repair system component
Database Link	Entrez Gene 5395 Human UniProt ID P54278
Background	Swiss-Prot Acc.P54278.Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.
Synonyms	HNPCC4; H_DJ0042M02.9; PMS2CL; PMSL2
Reference Data

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