PAK3 Rabbit Monoclonal Antibody [Clone ID: R04-2A4]

Specifications

Product Data
Clone Name	R04-2A4
Applications	IF, IHC, IP, WB
Recommend Dilution	WB: 1/2000-1/10000 IHC: 1/20-1/100 ICC/IF: 1/50 IP: 1/20
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser144 of human PAK1 (Phosphorylated)
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 61 kDa; Observed MW: 61 kDa
Database Link	UniProt ID O75914
Background	Swiss-Prot Acc.O75914.Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.
Reference Data

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