NELFE Rabbit Monoclonal Antibody [Clone ID: R02-7E5]

Specifications

Product Data
Clone Name	R02-7E5
Applications	IHC, IP, WB
Recommend Dilution	WB: 1/1000 IHC: 1/20 IP: 1/20
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	Recombinant protein of human NELFe
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 43 kDa; Observed MW: 43 kDa
Gene Name	negative elongation factor complex member E
Database Link	Entrez Gene 7936 Human UniProt ID P18615
Background	Swiss-Prot Acc.P18615.Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.
Synonyms	D6S45; NELF-E; RD; RDBP; RDP
Reference Data

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