H3FT (HIST3H3) Rabbit Monoclonal Antibody [Clone ID: R03-7K5]

Specifications

Product Data
Clone Name	R03-7K5
Applications	IF, IHC, WB
Recommend Dilution	WB: 1/1000 IHC: 1/50 ICC/IF: 1/50
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser28 of human Histone H3 (Phosphorylated)
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 15 kDa; Observed MW: 15 kDa
Gene Name	histone cluster 3, H3
Database Link	Entrez Gene 8290 Human UniProt ID P68431
Background	Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).CautionThe original paper reporting lysine deamination at Lys-5 by LOXL2 has been retracted due to inappropriate manipulation of figure data (PubMed:22483618, PubMed:27392148). However, this modification was confirmed in a subsequent publication (PubMed:27735137).
Synonyms	H3.4; H3/g; H3/t; H3FT; H3t; MGC126886; MGC126888
Reference Data

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