Histone H2A.X (H2AFX) Rabbit Monoclonal Antibody [Clone ID: R09-4A7]

Specifications

Product Data
Clone Name	R09-4A7
Applications	IP, WB
Recommend Dilution	WB: 1/2000 IP: 1/20
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser139 of human gamma H2A.X (Phosphorylated)
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 15 kDa; Observed MW: 15 kDa
Gene Name	H2A histone family member X
Database Link	Entrez Gene 3014 Human UniProt ID P16104
Background	Swiss-Prot Acc.P16104.Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Synonyms	H2A.X; H2A/X; H2AX
Reference Data

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