Caspase 8 (CASP8) Mouse Monoclonal Antibody [Clone ID: 4C2-3G4-5G7]

Specifications

Product Data
Clone Name	4C2-3G4-5G7
Applications	IF, IHC, WB
Recommend Dilution	WB: 1/1000-2000 IHC: 1/200-500 ICC: 1/200
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Protein of Caspase-8
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Observed MW (kDa):43,57
Gene Name	caspase 8
Database Link	Entrez Gene 841 Human UniProt ID Q14790
Background	Swiss-Prot Acc.Q14790.Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Synonyms	ALPS2B; CAP4; Casp-8; FLICE; FLJ17672; MACH; MCH5; MGC78473; OTTHUMP00000163720
Reference Data

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