ATP5F1B Rabbit Monoclonal Antibody [Clone ID: R01-4G1]

Specifications

Product Data
Clone Name	R01-4G1
Applications	IF, IHC, IP, WB
Recommend Dilution	WB: 1/2000-1/10000 IHC: 1/20-1/100 ICC/IF: 1/50 IP: 1/20-1/50
Reactivity	Human, Mouse, Rat, Monkey
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic peptide of human ATPB
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 57 kDa; Observed MW: 52 kDa
Gene Name	ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Database Link	Entrez Gene 506 Human UniProt ID P06576
Background	Swiss-Prot Acc.P06576.Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Synonyms	ATPMB; ATPSB; MGC5231
Reference Data

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