GAPDH Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB:1:5000~1:20000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Formulation	1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1 mg/mL
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Conjugation	Unconjugated
Predicted Protein Size	~ 36 kDa
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	Entrez Gene 2597 Human UniProt ID P04406
Background	Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.
Synonyms	G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432
Note	GAPDH polyclonal antibody detects endogenous levels of GAPDH protein. Not applicable to tissue.
Reference Data

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