ANP (NPPA) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against α-Atrial Natriuretic Factor. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Bovine, Human, Porcine
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Ser-Leu-Arg-Arg-Ser-Ser-Cys-Phe-Gly-Gly-Arg-Ile- Asp-Arg-Ile-Gly-Ala-Gln-Ser-Gly-Leu-Gly-Cys-Asn-Ser-Phe-Arg-Tyr-OH, (Disulfide bond) coupled to carrier protein.
Formulation	This polyclonal antibody is supplied as a lyophilized powder. The powder should be rehydrated with 50ml of RIA buffer. Upon reconstitution to 50ml total volume, the solution contains 0.1M sodium phosphate buffer (pH 7.4), 0.05M NaCl, 0.1% BSA, 0.01% NaN3, and 0.1% Triton X-100. Store at 4° - 8°C. This should ensure antibody stability for approximately one month.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	natriuretic peptide A
Database Link	Entrez Gene 4878 Human UniProt ID P01160
Background	Atrial Natriuretic Factor (ANF) is a 28 amino acid peptide hormone secreted mainly by the heart atria in response to atrial stretch. ANF acts on the kidney to increase sodium excretion and glomerular filtration rate (GFR), to antagonize renal vasoconstriction, and to inhibit renin secretion. This antibody was generated by immunization of guinea pigs with Atrial Natriuretic Factor (1-28) coupled to a carrier protein.
Synonyms	ANF; ANP; ATFB6; atriopeptin; cardionatrin; CDD-ANF; PND; prepronatriodilatin
Reference Data

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