EGF Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against Epidermal Growth Factor. Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His-Asp- Gly-Tyr-Cys-Leu-His-Asp-Gly-Val-Cys-Met-Tyr-Ile-Glu-Ala-Leu-Asp-Lys- Tyr-Ala-Cys-Asn-Cys-Val-Val-Gly-Tyr-Ile-Gly-Glu-Arg-Cys-Gln-Tyr-Arg- Asp-Leu-Lys-Trp-Trp-Glu-Leu-Arg-OH (Disulfide bonds between Cys⁶ and Cys²⁰/Cys¹⁴ and Cys³¹/Cys³³ and Cys⁴²) coupled to a carrier protein.
Formulation	Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	epidermal growth factor
Database Link	Entrez Gene 1950 Human UniProt ID P01133
Background	Epidermal Growth Factor (EGF) stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53- amino acid peptide hormone that stimulates cells to divide. This antibody was generated by immunization of rabbits with Epidermal Growth Factor coupled to a carrier protein.
Synonyms	beta-urogastrone; HOMG4; URG
Reference Data

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