Endothelin 1 (EDN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	This antibody has been tested and validated in ELISA against Big Endothelin-1 Fragment (22-38). Other applications like immunohistochemistry (IHC), FACS or Western Blot may work as well. Optimal dilutions should be determined by the end user.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys- Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-Val-Asn-Thr-Pro-Glu-His-Val- Val-Pro-Tyr-Gly-Leu-Gly-Ser-Pro-Arg-Ser-OH (Disulfide bridge: 1 - 15 and 3 - 11) coupled to carrier protein.
Formulation	Protein A affinity purified from antiserum, lyophilized, packaged under nitrogen. Reconstitute by adding 0.2ml distilled water. This stock solution contains 2mg/ml IgG, phosphate buffer saline pH 7.4 (PBS), and 0.02% (w/v) Thimerosal as a preservative.
Concentration	N/A
Conjugation	Unconjugated
Storage Condition	Original vial: at least one year at 4° - 8°C from date of delivery. Minimize repeated thawing and freezing of the antiserum by freezing aliquots at -20°C or below.
Gene Name	endothelin 1
Database Link	Entrez Gene 1906 Human UniProt ID P05305
Background	Big Endothelin-1 (Big ET), is a 38-amino-acid peptide and biological precursor of the potent vasoconstrictor Endothelin-1. It is cleaved to yield endothelin-1 and the C-terminal fragment (22-38) via the activity of a membrane-bound metalloproteinase called endothelin-converting enzyme (ECE). Endothelin is produced by several cell types including vascular endothelial cells, Mesangial cells and kidney cells. Big Endothelin-1 can be hydrolyzed by chymase to generate endothelin 1 (1-21) in vitro. This antibody was generated by immunization of rabbits with Big Endothelin-1 Fragment (22- 38) coupled to a carrier protein.
Synonyms	ET1; HDLCQ7; PPET1; Preproendothelin-1
Reference Data

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