ATP4A Monoclonal Antibody [Clone ID: PPC19]

Specifications

Product Data
Clone Name	PPC19
Applications	ELISA, IHC, WB
Recommend Dilution	Each lot of this antibody has been tested and validated for immunohistochemistry on formalin-fixed paraffin sections (IHC-P). Approximate working dilution for IHC: Paraffin sections: 4-8 µg/ml (1:50-1:100); microwave pretreatment for antigen retrieval is recommended.
Reactivity	Pig
Clonality	Monoclonal
Immunogen	Porcine stomach extract.
Specificity	Pig: parietal (oxyntic) cells Other: not tested Epitope: The antibody reacts with formaldehyde-treated porcine gastric H,K-ATPase. The epitope has not been further characterized. Distribution: Tissue sections: porcine gastric parietal (oxyntic) cells of the fundic gland region
Formulation	State: Affinity purified from cell culture supernatant, lyophilized, packaged under nitrogen. Buffer: Phosphate buffered saline pH 7.2 (PBS), 6 mg/ml BSA as a stabilizer and 0.05% (v/v) Kathon CG as a preservative.
Reconstitution Method	Reconstitute by adding 0.5 ml distilled water.
Concentration	0.4 mg/ml IgG after reconstitution
Conjugation	Unconjugated
Storage Condition	Original vial: 1 year at 4° - 8°C. Minimize repeated thawing and freezing of the stock solution.
Database Link	Entrez Gene 397552 Pig UniProt ID P19156
Background	Monoclonal antibody PPC19 recognizes the porcine gastric H,K-ATPase. This enzyme catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane and is thus responsible for acid production in the stomach. The functional proton pump is actually a heterodimer consisting of a 114kDa alpha subunit with catalytic activity, and a 33kDa stabilizing beta subunit. Of the various cell types that form the gastric mucosa, only parietal (oxyntic) cells of the fundic gland region possess this enzyme. Of pathological significance, their dysfunction induced by H. pylori infection leads to atrophic gastritis. This antibody was produced in vitro in bioreactors.
Synonyms	ATP6A
Reference Data

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