Neutrophils Mouse Monoclonal Antibody [Clone ID: PM1 Biotin]

Specifications

Product Data
Clone Name	PM1 Biotin
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC). Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:4000) IHC, paraffin sections: 1µg/ml (1:400) microwave pretreatment for antigen retrieval is recommended. Optimal dilutions should be determined by the end user.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine lung extract
Specificity	Pig: Neutrophils Other species: reacts with human neutrophils. Epitope: The epitope has not been characterized Distribution: Tissue sections: The antigen of 21kDa is associated with neutrophils in various swine tissues, e.g. in the red pulp of the spleen, and also with neutrophils in blood smears.
Formulation	Affinity purified, biotinylated, lyophilized; reconstitute by adding 0.5ml distilled water. This stock solution contains 0.4mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 5mg/ml bovine serum albumin (BSA) as a stabilizer and 0.05% Kathon CG as a preservative.
Concentration	N/A
Conjugation	Biotin
Storage Condition	Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Background	Monoclonal antibody PM1 recognizes neutrophils in various organs and in blood smears. The antigen is located in both the membrane and the cytoplasm. Western Blotting of kidney or spleen extracts reveals a band at 21kDa after SDS-PAGE under non-reducing conditions. No band is visible under reducing conditions.
Reference Data

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