Lymphocyte Activation Gene 3 (LAG3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1-2µg/mL. IHC: 5µg/mL.Immunoflorescence start at 20µg/mL.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	LAG3 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human LAG3. The immunogen is located within amino acids 110 - 160 of LAG3.
Formulation	LAG3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	LAG3 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 55 kDa; Observed: 54 kDa
Gene Name	lymphocyte activating 3
Database Link	NP_002277 Entrez Gene 3902 Human UniProt ID P18627
Background	The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).
Synonyms	CD223; FDC; LAG-3
Note	LAG3 antibody can be used for Western blot at 1 - 2 µg/mL. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.
Reference Data

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