RHOA Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Dot, WB
Recommend Dilution	WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
Reactivity	Human, Rat, Mouse, Chicken, Canis
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to the C-term of epitope KKKSG at a phosphorylation Ser188 of human Rho protein
Formulation	This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Purification	The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	~24 kDa
Gene Name	ras homolog family member A
Database Link	NP_001655 Entrez Gene 11848 MouseEntrez Gene 117273 RatEntrez Gene 387 Human UniProt ID P61586
Background	Ras homolog gene family consists of RhoA, RhoB, RhoC proteins. These proteins interact directly with protein kinase (Rho kinase) to serve as downstream effectors of the activated GTPase. The Rho proteins are involved in the regulation of the actin cytosekeleton. Activation of Rho to the GTP bound state promotes formation of stress fibers and is required for the formation and maintenance of focal adhesions. Pathways stimulated by cytokines, hormones and various forms of stress utilize p21 proteins of the Rho family2. Possible mediators for these responses include protein kinase such as p120PKN, and p150, ROKa/Rho-A kinase, myosin light chain (MLC), phosphatase and PIP 5-kinase.3
Synonyms	ARH12; ARHA; RHO12; RHOH12
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Adherens junction, Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Pathways in cancer, Regulation of actin cytoskeleton, T cell receptor signaling pathway, TGF-beta signaling pathway, Tight junction, Vascular smooth muscle contraction, Wnt signaling pathway

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