Mtor Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to the C-terminus of human mTOR protein. This sequence is identical among human, rat, mouse, chicken, dog and bovine species.
Formulation	This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Purification	The Rabbit IgG is purified by Epitope Affinity Purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	290 kDa
Gene Name	mechanistic target of rapamycin
Database Link	NP_063971 Entrez Gene 2475 HumanEntrez Gene 56717 MouseEntrez Gene 56718 Rat UniProt ID P42346
Background	The mammalian target of rapamycin (mTOR), also named FKBP12 rapamycin-associated protein (FRAP/RAFT/RAPT/SEP), is a Ser/Thr protein kinase that plays a crucial role in a nutrient-sensitive signalling pathway that regulates cell growth. TOR signalling is potently inhibited by rapamycin, through the direct binding of a FK506-binding protein 12 (FKBP12)/rapamycin complex to the TOR FRB domain, a segment amino terminal to the kinase catalytic domain. mTOR is involved in the regulation of cell growth through initiation of gene translation in response to nutrients such as amino acids (mainly leucine), growth factors, insulin and mitogens. mTOR initiates translation by activating the ribosomal p70S6k protein kinase (S6K1) and by inhibiting the eIF4E inhibitor 4E-BP1. mTOR is phosphorylated at serine 2448 via the phosphatidylinositol 3-kinase (PI3K)/Akt pathway and is autophosphorylated at serine 2481.
Synonyms	FLJ44809; FRAP; FRAP1; FRAP2; KIAA1303; RAFT1; RAPT1; RAPTOR; RPTOR
Reference Data

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