beta Catenin (CTNNB1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to the epitope –KRLSVEL- with a single phosphorylation site Ser675 of human b-catenin.
Formulation	This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Purification	The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	88 kDa
Gene Name	catenin beta 1
Database Link	NP_001091679 Entrez Gene 12387 MouseEntrez Gene 84353 RatEntrez Gene 1499 Human UniProt ID P35222
Background	Beta-catenin, a cytosolic, 88 kDa, 781 amino acid protein, belongs to the β-catenin family. The N-terminus domain, contains the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin(s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, β-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3β and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates β-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280, S675, etc. Phosphorylation of β-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3β activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3β, allowing unphosphorylated β-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.
Synonyms	armadillo; CTNNB; MRD19
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
Protein Pathways	Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway

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