H3FA (HIST1H3A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Dot, ELISA, IF, IP, WB
Recommend Dilution	ChIP (1 µl/ChIP); ELISA (1:100 ?? 1:500); Dot blotting (1:20,000); Western blotting (1:250 - 1:500); Immunofluorescence (1:200); Immunoprecipitation (5 µl/IP)
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen for anti-H3K27me3S28p antibody: histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p), using a KLH-conjugated synthetic peptide.
Concentration	lot specific
Purification	Whole antiserum from rabbit containing 0.05% azide.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	histone cluster 1, H3a
Database Link	NP_003520 Entrez Gene 8350 Human UniProt ID P68431
Background	Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Phosphorylation of H3 on serine 28 is increased during mitosis.
Synonyms	A; H3; H3FA
Reference Data
Protein Pathways	Systemic lupus erythematosus

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