EPHX2

Specifications

Product Data
Applications	IHC
Recommend Dilution	IHC IHC-P (10 - 20 µg/ml)
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic 18 amino acid peptide from internal region of human EPHX2. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon (94%); Monkey (83%).
Specificity	Human EPHX2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except RHOBTB2 (44%), RHOBTB1 (44%).
Formulation	PBS, 0.1% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity purified
Conjugation	Unconjugated
Storage Condition	Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Gene Name	epoxide hydrolase 2
Database Link	NP_001970 Entrez Gene 2053 Human UniProt ID P34913
Background	Epoxide Hydrolase 2 antibody TA341174 is an unconjugated rabbit polyclonal antibody to human Epoxide Hydrolase 2 (EPHX2) (Internal). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Synonyms	EPHX2, CEH, Epoxide hydratase, Epoxide hydrolase, soluble, Epoxide hydrolase 2, cytosolic, SEH, Soluble epoxide hydrolase
Note	Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.
Reference Data
Protein Pathways	Arachidonic acid metabolism, Metabolic pathways

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