LIF Rat Monoclonal Antibody [Clone ID: 39N7D10]

Specifications

Product Data
Clone Name	39N7D10
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry, Immunohistochemistry-Paraffin: 5 ug/mL, Western Blot: 3-5ug/ml~, Immunocytochemistry/ Immunofluorescence
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	leukemia inhibitory factor
Database Link	NP_002300 Entrez Gene 16878 MouseEntrez Gene 3976 Human UniProt ID P15018
Background	Lif has long been recognized as a cytokine which promotes murine germ cell growth in the absence of feeder cell layers in mouse cells (1-3)Characterized initially as an inhibitor of differentiation in M1 murine monocytic leukemia lines (hence the name Leukemia Inhibitory Factor),Lif is now recognized as a key factor for maintaining stem cells in the undifferentiated state- at least in mouse embryonic stem cells (4)Acting through the Lif receptor and the induced activation of STAT3, murine ESCs can be maintained in vitro in undifferentiated stateHowever, human ESCs, although functionally stimulated with Lif, are not similarly maintained as undifferentiatedThis illustrates a different functional program for Lif in mice and humans in maintenance of stem cellsAn IL-6 family member, Lif is an important pleiotropic cytokine with activity on cells of hematopoietic and non-hematopoietic origin.
Synonyms	CDF; DIA; HILDA; MLPLI
Note	The Lif protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. In IHC-P application, 1M EDTA buffer pH 9.0 was used for antigen retrieval (citrate buffer pH 6.0 did not work for this target).
Reference Data
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

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