CLEC9A Mouse Monoclonal Antibody [Clone ID: 14N8D7]

Specifications

Product Data
Clone Name	14N8D7
Applications	WB
Recommend Dilution	Western Blot: 1-3ug/ml
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	A portion of amino acids 50-130 of human CLEC9A was used as the immunogen for the antibody. 60 74
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	C-type lectin domain family 9 member A
Database Link	NP_997228 Entrez Gene 283420 Human UniProt ID Q6UXN8
Background	CLEC9A is a group V CTLR (C-type lectin-like receptor) that functions as an activation receptor and is expressed on myeloid lineage cellsThis protein is also expressed at the cell surface and acts as glycosylated dimers which mediate endocytosis, but not phagocytosis (Poulin LF et al, 2010). It facilitates antigen uptake and presentation, and may provide a suitable target for antibody-mediated antigen delivery. CLEC9A is important for conveying information from necrotic cells to T-cells and defines a pathway by which adaptive immune responses can be initiated in the absence of infection (Huysamen C et al, 2008). It recruits Syk to mediate sensing of necrosis by the principal DC subset involved in regulating cross priming to cell-associated antigens (Sancho D et al, 2009). Signaling via this kinase occurs through a novel pathway involving CARD9 which induce a variety of cellular responses including the induction of cytokines (such as TNF, IL-6, IL-10, IL-23, and IL-2), the respiratory burst, and the production of Arachidonic acid (Caminschi I et al, 2008). In addition, it also induces TH17 (T-helper type 17) adaptive responses in vivo. Expression of CLEC9A is highly restricted in peripheral blood, being detected only on BDCA3+ DCs and on a small subset of CD14+ CD16- monocytes. CLEC9A serves as a new marker to distinguish subtypes of DCs. Targeting antigens to DCs with antibodies to CLEC9A is a promising strategy to enhance the efficiency of vaccines, even in the absence of adjuvants (Sancho D et al, 2008).
Synonyms	CD370; DNGR-1; DNGR1; UNQ9341
Reference Data
Protein Families	Transmembrane

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