53BP1 (TP53BP1) Mouse Monoclonal Antibody [Clone ID: 6B3E10]

Specifications

Product Data
Clone Name	6B3E10
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry-Paraffin: 1:200 - 1:1000, Immunohistochemistry: 1:200 - 1:1000, Flow Cytometry: 1:200 - 1:400, Western Blot: 1:500 - 1:2000, Immunocytochemistry/ Immunofluorescence
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Partial recombinant human 53BP1 (between residues 500-800) expressed in E. coli [Uniprot Q12888]
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	213 kDa
Gene Name	tumor protein p53 binding protein 1
Database Link	NP_005648 Entrez Gene 7158 Human UniProt ID Q12888
Background	53BP1 (p53 binding protein 1) plays a key role in response to DNA damage, checkpoint signaling during mitosis and enhancing TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 now has been established as a substrate for ATM (ataxia telangiectasia mutated) signaling and that it relocalizes to discrete foci overlapping with gamma H2AX (phosphorylated histone H2AX); demarcating DNA double strand breaks (DSBs) sites following exposure to radiation. 53BP1 functions downstream of gamma H2AX-dependent hierarchy of proteins that collectively establish IRIF (ionizing radiation induced foci) at DSBs; this hierarchy includes Mre11/Rad50/NBS1 (MRN complex), ATM, MDC1, RNF8, RNF168 and HERC2. With the exception of ATM, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-PK (DNA-dependent kinase), all of these proteins are physically and functionally required to recruit 53BP1 to the DSB site. Briefly, this process involves DSB recognition by MRN, ATM activation, gamma H2AX-formation, MDC1-recruitment, MRN-retention (leading to further ATM-activation and gamma H2AX spreading) and RNF8/RNF168/HERC2-mediated histone H2A and H2AX mono and poly-ubiquitination.
Synonyms	53BP1; p202
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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