GLI2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC
Recommend Dilution	Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin: 1:200 - 1:300, Immunohistochemistry: 1:200 - 1:300, Immunocytochemistry/ Immunofluorescence: 1:50 - 1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human Gli2 protein (between residues 300-400) [UniProt P10070]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	GLI family zinc finger 2
Database Link	NP_005261 Entrez Gene 14633 MouseEntrez Gene 2736 Human UniProt ID P10070
Background	Gli2, also known as THP, is a transcription factor which binds DNA through zinc finger motifs. Belonging to the C2H2-Zinc finger type subclass of the Gli family, Gli2 is a mediator of Shh (sonic hedgehog) signaling, a pathway that is aberrantly activated in many types of cancers. Specifically, it has been found that the anti-apoptotic protein BCL-2 is up regulated by Gli2, leading to carcinogenesis. The protein encoded by the Gli2 gene localizes to the cytoplasm and activates patched (Drosophila homolog) gene expression, where it has been thought to play an important role during embryogenesis. Mutations in the Gli2 gene are found in various syndromes, including Pallister-Hall Syndrome, Greig cephalopolysyndactyly syndrome, and preaxial polydactyl type IV. Recently, the interaction between the hedgehog signaling pathway and Gli2 has been implicated in medullary thyroid carcinoma (PMID: 23410206).
Synonyms	CJS; HPE9; PHS2; THP1; THP2
Note	In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
Protein Pathways	Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer

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