TORC2 (CRTC2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:20 - 1:100, Western Blot: 1.0 - 2.0 ug/ml, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:100
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of the human Torc2 protein (between residues 600-693) [UniProt Q53ET0]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	CREB regulated transcription coactivator 2
Database Link	NP_859066 Entrez Gene 74343 MouseEntrez Gene 200186 Human UniProt ID Q53ET0
Background	CREB-regulated transcription coactivator 2 (CRTC2), also known as transducer of regulated cAMP response element-binding protein 2, Torc2, or transducer of CREB protein 2, is a transcriptional coactivator for both CREB and the human T-cell leukemia virus type I (HTLV-1). In its dephosphorylated state, Torc2 becomes an activator in the SIK/TORC signaling pathway, and acts as a central regulator of cAMP dependent gluconeogenic gene expression in the LKB1/AMPK/TORC2 pathway. While Torc2 is normally found in the nucleus, it is translocated to the cytoplasm upon phosphorylation. Torc2 is abundantly expressed in the thymus but can also be found in the spleen, ovary, lung and muscle (where it acts as an inducer of mitochondrial biogenesis). Current research suggests that Torc2 responds to plasma membrane stress by facilitating changes in cellular lipid synthesis, thus altering the composition of the plasma membrane (PMID: 22504275). Other research indicates that Torc2 plays a role in regulating cancer cell migration, invasion, and metastasis (PMID: 21339740).
Synonyms	TORC-2; TORC2
Note	This Torc2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 85 kDa. In ICC/IF cytoplasmic staining was observed in Hela cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data

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