MMP9 Mouse Monoclonal Antibody [Clone ID: 4A3]

Specifications

Product Data
Clone Name	4A3
Applications	ELISA, IHC, WB
Recommend Dilution	Western Blot: 1:500-1:1000, ELISA: 1:100 - 1:2000, Immunohistochemistry: 1:100-1:200, Immunohistochemistry-Paraffin: 1:100-1:200
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide from the N-terminus of human MMP9 [Swiss-Prot# P14780]
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	matrix metallopeptidase 9
Database Link	NP_004985 Entrez Gene 81687 RatEntrez Gene 4318 Human UniProt ID P14780
Background	MMP9, also known as Matrix metallopeptidase 9 or gelatinase B is a member of the matrix metalloproteinase (MMP) family. The MMPs are zinc-containing enzymes involved in the degradation of the extracellular matrix in normal physiological processes including embryonic development, reproduction and tissue remodeling. Most MMP family members are secreted as inactive proproteins that become activated when cleaved by extracellular proteinases. The proenzyme MMP9 is thought to be a 92 kDa protein. Processing of the precursor MMP9 yields different active forms, two of which were identified in Duncan et al 1998 (PMID: 9851689) to be 82 kDa and 63 kDa proteins. The 82 kDa protein results from cleavage at the Arg87-Phe88 bond. And the 63 kDa protein is caused by a further cleavage to the 82 kDa protein within the C-terminus domain between Arg527-Gly528. The main 63 kDa protein produced thus has the N-terminus of activated MMP9 and has a cleaved C-terminus. Antibodies specific to the active forms of MMP9 can be useful tools for studying important pathological processes such as tumor invasion, metastasis and arthritis.
Synonyms	CLG4B; GELB; MANDP2; MMP-9
Note	This MMP9 Antibody (4A3) is useful for Western Blot, ELISA and Immunohistochemistry on paraffin embedded sections. In Western Blot a band can be seen at 82 or 63 kDa, representing the active forms of MMP9. In IHC, cytoplasmic and extracellular staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Protease
Protein Pathways	Bladder cancer, Leukocyte transendothelial migration, Pathways in cancer

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