Amyloid Precursor Protein (APP) Mouse Monoclonal Antibody [Clone ID: MOAB-2]

Specifications

Product Data
Clone Name	MOAB-2
Applications	Dot, ELISA, ICC/IF, IHC, Immunoblotting, IP, WB
Recommend Dilution	Immunohistochemistry Free-Floating, Dot Blot, Immunoblotting, Immunohistochemistry-Paraffin: 1:200-1:1000, Immunohistochemistry: 1:200-1:1000, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence: 1:200-1:500, Immunoprecipitation: 1:200-1:1000, Western Blot: 1:1000-1:5000, ELISA: 1:100-1:1000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human beta Amyloid 42
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	amyloid beta precursor protein
Database Link	NP_958816 Entrez Gene 11820 MouseEntrez Gene 54226 RatEntrez Gene 351 Human UniProt ID P05067
Background	Beta Amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the Amyloid precursor protein (APP). Beta Amyloid is known as the main component of amyloid plaques, which are deposits found in the brains of patients with Alzheimer's disease. The form(s) of beta Amyloid associated with the pathology characteristic of Alzheimer's disease still remains unclear. Currently, controversy exists as to the existence of intraneuronal beta Amyloid. Research indicates that intraneuronal beta Amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Many commercially available beta Amyloid antibodies cross react with APP or APP C-terminal fragments. This MOAB-2 antibody is specific for beta Amyloid but not APP. MOAB-2 is a pan-specific beta Amyloid antibody that detects unaggregated, oligomeric and fibrillar forms of beta Amyloid. This is important because beta Amyloid is known to aggregate and form a variety of potentially toxic conformations. MOAB-2 displays strong extracellular beta Amyloid immunoreactivity, and so detects classic plaque pathology. MOAB-2 is selective for Abeta 42 over Abeta 40. This is also an advantage as the 42 amino acid form of beta Amyloid is likely the toxic form. MOAB-2 demonstrates strong intraneuronal immunoreactivity in both human and beta Amyloid-Tg mouse tissue and has the potential to aid future research into the functional importance of intraneuronal beta Amyloid.
Synonyms	AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2
Note	This beta Amyloid Antibody (MOAB-2) is useful for Immunocytochemistry/Immunofluorescence, ELISA, Immunoprecipitation, Western Blot, and Immunohistochemistry on paraffin-embedded sections. In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931).
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Alzheimer's disease

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