Fibronectin (FN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence: 1:400, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:400, Simple Western: 1:100, Immunohistochemistry: 1:400
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made toward the C-terminal region of the human Fibronectin protein (within residues 2250-2300). [Swiss-Prot P02751]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	fibronectin 1
Database Link	NP_997639 Entrez Gene 14268 MouseEntrez Gene 2335 Human UniProt ID P02751
Background	Fibronectin EDA (FN-EDA, FN, fibronectin 1) is a glycoprotein that is involved in cell adhesion as well as cell migration processes such as embryogenesis, wound healing, host defense and metastasis. Fibronectin appears to be an important factor in the ability of cancerous cells to metastasize by inducing matrix metalloproteinases (MMPs). In a study of human laryngeal carcinoma cells, researchers showed that FN-induced expression of MMP-9 increased the DNA binding activity of transcription factors NF-kappa-B, Activator protein-1 and Specificity protein-1 to the promoter region (PMID: 20638438). Additionally, a study of renal cell cancer showed that the mRNA expression of fibronectin 1 was higher in patients with renal cell cancer as compared to patients with healthy kidneys. This disparity in mRNA expression levels could potentially be used to determine the aggressiveness of renal cell cancer as well as indicate the progression of the disease in patients (PMID: 20860816). Furthermore, a knockdown study of endogenous FN in the A431-III cell line showed a significant decrease of the migratory and invasive activity of the cells as well as an inhibition of secreted MMP-9 (PMID: 21036738).
Synonyms	CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF
Note	This Fibronectin antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and IHC-paraffin embedded sections. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In Western Blot, a band is seen ~262 kDa representing Fibronectin. In IHC-P, staining was observed in the cytoplasm and extracellular space of mouse prostate tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer

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