CHORDC1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:150, Immunocytochemistry/ Immunofluorescence: 1:100-1:400, Western Blot: 1:2500, Immunohistochemistry-Paraffin: 1:150
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human CHORDC1 protein (between residues 100-250) [UniProt Q9UHD1]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cysteine and histidine rich domain containing 1
Database Link	NP_001137545 Entrez Gene 66917 MouseEntrez Gene 26973 Human UniProt ID Q9UHD1
Background	Cysteine and histidine-rich domain containing 1 (CHORDC1; CHP1) is a member of a novel family of highly conserved proteins. These proteins are factors in plant disease resistance, as well as animal development. Mammalian Chp-1 is a novel Hsp90-interacting protein that contains two CHORD domains and one CS domain. CHP-1 binds to the ATPase domain of Hsp90, but this interaction does not utilize ATP and ATPase- coupled conformational change of Hsp90 (PMID: 15642353). High ADP:ATP ratios in cell lysates appears to promote the interaction between CHP1 and Hsp90 (PMID: 19875381). Additionally, CHP-1 has been shown to be 63% homologous to Melusin; a muscle specific protein necessary for heart hypertrophy caused by mechanical burden (PMID12965203).
Synonyms	CHP1
Note	This CHORDC1 antibody is useful for Western Blot where a band is observed ~37kDa, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data

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