NOTCH1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Simple Western: 1:50, Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Western Blot: 2 ug/ml, Immunohistochemistry-Paraffin: 1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of the human NOTCH1 protein (between residues 2300-2350) [UniProt P46531]
Formulation	PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	notch 1
Database Link	NP_060087 Entrez Gene 18128 MouseEntrez Gene 4851 Human UniProt ID P46531
Background	Notch1 is a single-pass transmembrane protein that serves as the receptor for the Delta1, Jagged1 and Jagged2 ligands. Activation of Notch1 causes the cytoplasmic domain to be cleaved, releasing the notch intracellular domain (NICD) which forms a complex with RBPJ/RBPSUH and subsequently influences transcription of its target genes. Because the majority of Notch1 ligands are transmembrane proteins, Notch1 is primarily triggered by cell-to-cell contact and employs lateral inhibition mechanisms to influence local populations of cells. The Notch1 pathway plays a pivotal role in development, including cell-fate determination, proliferation, and cell contact-dependent signaling. Aberrant activation of the Notch1 pathway has been associated with numerous diseases such as bicuspid aortic valve and cancer-- in particular certain leukemias.
Synonyms	AOS5; AOVD1; hN1; TAN1
Note	This Notch1 antibody is useful for Western Blot and Immunohistochemistry-Paraffin. In Western Blot, a band is seen ~120 kDa representing the cleaved form of Notch1 peptide. In IHC-P, staining of nucleus and cell membrane was observed in human kidney carcinoma tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway
Protein Pathways	Dorso-ventral axis formation, Notch signaling pathway, Prion diseases

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