Dux4 (DUX4L1) Mouse Monoclonal Antibody [Clone ID: P4H2]

Specifications

Product Data
Clone Name	P4H2
Applications	ELISA, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 1:100-1:200, Immunocytochemistry/ Immunofluorescence: 1:100-1:200, Western Blot: 0.5-2.0ug/ml, Immunohistochemistry-Paraffin: 1:100-1:200, Immunohistochemistry-Frozen: 1:100-1:200, Simple Western: 1:10, ELISA
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to the C-terminus of human DUX4. [Swiss-Prot# Q9UBX2]
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	52 kDa
Gene Name	double homeobox 4 like 1
Database Link	NP_149418 Entrez Gene 22947 Human
Background	DUX4 (also known as Double homeobox 4) is the leading candidate causative gene for facioscapulohumeral dystrophy (FSHD), a degenerative skeletal muscle disease and one of the most common muscular dystrophies. FSHD is caused by the deletion of a subset of D4Z4 macrosatellite repeats on chromosome 4. Each repeat contains a retrogene encoding the double-homeobox factor DUX4. DUX4 expression is epigenetically suppressed in differentiated tissues and the residual DUX4 transcripts are spliced to remove the carboxyterminal domain that has been associated with cell toxicity. In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle, and possibly other differentiated tissues, and results in a small percentage of cells expressing relatively abundant amounts of the full-length DUX4 mRNA and protein. [Snider et al. (2010) PLoS Genetics.6(10): e1001181]
Synonyms	DUX10
Note	This DUX4 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen sections, Immunohistochemistry paraffin embedded sections and Western Blot. In Western blot, a band can be seen at ~52 kDa.
Reference Data

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