SIRT1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Knockdown Validated, Simple Western: 1:100, Immunohistochemistry: 1:150, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:150, Knockout Validated
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A genomic peptide made to an internal region of the human SIRT1 protein (within residues 400-550). [Swiss-Prot Q96EB6]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	sirtuin 1
Database Link	NP_036370 Entrez Gene 23411 Human UniProt ID Q96EB6
Background	SIRT1 (mammalian homologue of silent information regulator 2), is a NAD-dependent class III deacetylase which belongs to mammalian sirtuin family and generates enzymatic activity in the presence of NAD+. SIRT1 is primarily localized in the nucleus and deacetylates not only histones but also a number of other transcription factors/cofactors, such as p53, FOXO (forkhead), PPAR- gamma coactivator-1 alpha, and NFkB, thus affecting the critical cellular pathways involved in stress response. SIRT1 plays an important role in the energy metabolism by interacting with PPAR-gamma and inhibiting its transcriptional activity towards genes mediating fat storage through docking with its cofactors. SIRT1 also plays an essential function in mediating cellular survival by deacetylating substrates such as FOXO, p53 , DNA repair factor Ku70 and NFkB. SIRT1 has been shown to get upregulated in response to stress-induced cell death, especially upon oxidative stress and calorie restriction, and SIRT1 knockout mice fail to extend lifespan induced by calorie restriction. Moreover, expression of SIRT1 gets increased to promote neuronal survival in Alzheimer's disease model as well as in primary neurons subjected to neurotoxic insult.
Synonyms	SIR2; SIR2alpha; SIR2L1
Note	This SIRT1 antibody is useful in Immunocytochemistry/Immunfluorescence, Immunhistochemistry paraffin embedded sections and Western blot. In Western blot a band is seen ~105 kDa.
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors

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