CCR2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:50, Western Blot: 2 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Immunohistochemistry: 5 ug/ml, Flow Cytometry: 5 ug/ml
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to an N-terminal portion of the human CCR2 protein (within residues 20-100). [Swiss-Prot# P00338]
Formulation	PBS, 30% glycerol, 0.1% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Database Link	NP_000639 Entrez Gene 1231
Background	CCR2 is a major chemokine receptor expressed on monocytes and its activation through its ligands MCP1, MCP2, MCP3 and MCP4 (systematic names CCL2, CCL8, CCL7 and CCL13, respectively) stimulates the migration of monocytes across the vascular wall into tissues wherein they facilitate chronic inflammation. CCR2 is a member of GPCR1 family and is up-regulated by CREB3. It is a multi-pass membrane protein found in cell membrane, and besides monocytes, it is also found on other cell types like NK cells, macrophages, immature dendritic cells, gamma delta T cells, and activated T cells (including Th17 cells). There are two alternatively spliced forms of CCR2: CCR2A - the major isoform expressed by mononuclear cells and vascular smooth muscle cells; and CCR2B - expressed predominantly by monocytes and activated NK cells. CCR2 has both pro-inflammatory (mediated by APC and T cells) and anti-inflammatory (mediated by regulatory T cells) activities. Besides inflammation, it has been implicated several pathologies including HIV-1/AIDS, autoimmune disorders (psoriasis, rheumatoid arthritis, and multiple sclerosis), pulmonary diseases (asthma, COPD), vascular disease and cancer (prostate cancer).
Synonyms	CC-CKR-2; CCR2A; CCR2B; CD192; CKR2; CKR2A; CKR2B; CMKBR2; MCP-1-R
Note	This CCR2 antibody is useful for Flow Cytometry, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. Some customers have also reported success using this antibody for WB on rat and human lysates.
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane

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