beta Actin (ACTB) Mouse Monoclonal Antibody [Clone ID: 8H10D10]

Specifications

Product Data
Clone Name	8H10D10
Applications	ELISA, FC, ICC/IF, Immunoblotting, WB
Recommend Dilution	Flow (Intracellular): 1:200 - 1:400, Western Blot: 1:1000-1:5000, Simple Western: 12.5, Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Immunoblotting
Reactivity	Human, Mouse, Rat, Chinese Hamster, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to amino-terminal residues of human Beta Actin, conjugated to KLH. [UniProt# P60709]
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	42 kDa
Gene Name	actin, beta
Database Link	NP_001092 Entrez Gene 11461 MouseEntrez Gene 81822 RatEntrez Gene 60 Human UniProt ID P60709
Background	Beta Actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because Beta Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to Beta Actin provide a specific and useful tool in studying the intracellular distribution of Beta Actin and the static and dynamic aspects of the cytoskeleton.
Synonyms	BRWS1; PS1TP5BP1
Note	This Beta Actin (8H10D10) antibody is useful in Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence and ELISA.
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis

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