Estrogen Related Receptor alpha (ESRRA) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry-Paraffin: 2 ug/ml, Immunohistochemistry: 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 2 ug/ml
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to an N-terminal portion of human Estrogen Related Receptor alpha (within residues 1-50). [Swiss-Prot# P11474]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	50 kDa
Gene Name	estrogen related receptor alpha
Database Link	NP_004442 Entrez Gene 26379 MouseEntrez Gene 2101 Human UniProt ID P11474
Background	Nuclear receptors are essential in embryonic development as well as for the regulation of adult physiological functions, and estrogen related receptor alpha (ERR-alpha) is one such receptors expressed in several tissues requiring high energy demand, including heart, skeletal muscle and brain. ERR-alpha binds to ERRE (ERR-alpha response element) containing a single consensus half-site, 5'-TNAAGGTCA-3'; to MCAD response element NRRE-1 for regulating MCAD promoter, and it also regulates lactoferrin gene promoter. ERR-alpha is an orphan nuclear hormone receptor capable of regulating transcription of genes involved in multiple cellular/physiological processes such as regulation of energy metabolism wherein it is co-activated by PPAR gamma and PGC-1 alpha to regulate several genes required for mitochondrial function. ERR-alpha regulates estrogen signaling and fatty acid oxidation/adaptative bioenergetic response, and is involved in cartilage/bone formation, macrophage activation and carcinogenesis. High ERR-alpha expression levels coupled with poor prognosis have been observed in various malignancies including colon, endometrium, ovary, prostate and breast cancers, and adrenocortical carcinoma.
Synonyms	ERR1; ERRa; ERRalpha; ESRL1; NR3B1
Note	This Estrogen Related Receptor alpha antibody is useful for Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. ERR alpha/ ESRRA localize mainly to the nucleus, but there are reports on its cytoplasmic presence also, especially in cells with high nuclear expression. In a feedback loop mechanism, it has been shown that ERK8 /MAPK15 interacts with ERR alpha to induce its CRM1-dependent translocation to cytoplasm for inhibiting its transcriptional activity via regulation of its cellular localization (PMIDs 21190936, 18070081).
Reference Data
Protein Families	Druggable Genome, Nuclear Hormone Receptor, Transcription Factors

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