LOX Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IP, Simple Western, WB
Recommend Dilution	Immunoprecipitation: 1:10-1:500, Flow Cytometry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:200, Western Blot: 0.5 ug/ml, Simple Western: 1:200
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to an internal portion of human LOX propeptide (within residues 118-168). [Swiss-Prot# P28300]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	lysyl oxidase
Database Link	NP_002308 Entrez Gene 24914 RatEntrez Gene 4015 Human UniProt ID P28300
Background	Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.
Synonyms	MGC105112
Note	This LOX propeptide antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation (PMID: 21536655) and Western Blot. In WB a band can be seen at ~35 kDa (glycosylated propeptide form) and at ~50 kDa (proenzyme form).
Reference Data
Protein Families	Druggable Genome, Secreted Protein

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