CDCA8 Rabbit Polyclonal Antibody
CNY 5,381.00
货期*
5周
规格
经常一起买 (2)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
CNY 300.00
CNY 1,430.00
Specifications
Product Data | |
Applications | ICC/IF, WB |
Recommend Dilution | Western Blot: 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:50-1:200 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Synthetic peptide made to an internal portion of human Borealin (within residues 200-250). [Swiss-Prot# Q53HL2] |
Formulation | PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Concentration | lot specific |
Purification | Immunogen affinity purified |
Conjugation | Unconjugated |
Storage Condition | Store at -20°C as received. |
Predicted Protein Size | 38 kDa |
Gene Name | cell division cycle associated 8 |
Database Link | |
Background | Encoded by gene CDCA8, borealin forms an important component of mitosis regulating chromosomal passenger complex (CPC complex - BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC) which is known for ensuring correct chromosome alignment/segregation and is required for chromatin-induced microtubule stabilization as well as spindle assembly. Borealin governs CPC to centromeric DNA and is a major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Besides associating with CPC complex subunits, borealin interact with SENP3, UBE2I and RANBP2 which are involved its sumoylation. Borealin is expressed in a cell cycle-dependent manner and the levels of endogenous borealin significantly increase during mitosis. TTK can phosphorylated borealin at Thr-88, Thr94, Thr-169 and Thr-230 and and mutation of S219 to alanine in borealin causes defects in mitotic progression advocating for the importance of phosphorylation in borealin function. Mutation of additional sites creates a form of borealin that is defective in localizing to the centromere, binding to Sgo1 and Sgo2, and in coordinating chromosome alignment. |
Synonyms | BOR; BOREALIN; DasraB; MESRGP |
Note | This Borealin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen ~38 kDa. |
Reference Data | |
Protein Families | Druggable Genome |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |
Customer
Reviews
Loading...