CDCA8 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, WB
Recommend Dilution	Western Blot: 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:50-1:200
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to an internal portion of human Borealin (within residues 200-250). [Swiss-Prot# Q53HL2]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	38 kDa
Gene Name	cell division cycle associated 8
Database Link	NP_060571 Entrez Gene 55143 Human UniProt ID Q53HL2
Background	Encoded by gene CDCA8, borealin forms an important component of mitosis regulating chromosomal passenger complex (CPC complex - BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC) which is known for ensuring correct chromosome alignment/segregation and is required for chromatin-induced microtubule stabilization as well as spindle assembly. Borealin governs CPC to centromeric DNA and is a major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. Besides associating with CPC complex subunits, borealin interact with SENP3, UBE2I and RANBP2 which are involved its sumoylation. Borealin is expressed in a cell cycle-dependent manner and the levels of endogenous borealin significantly increase during mitosis. TTK can phosphorylated borealin at Thr-88, Thr94, Thr-169 and Thr-230 and and mutation of S219 to alanine in borealin causes defects in mitotic progression advocating for the importance of phosphorylation in borealin function. Mutation of additional sites creates a form of borealin that is defective in localizing to the centromere, binding to Sgo1 and Sgo2, and in coordinating chromosome alignment.
Synonyms	BOR; BOREALIN; DasraB; MESRGP
Note	This Borealin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen ~38 kDa.
Reference Data
Protein Families	Druggable Genome

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