MCP1 (CCL2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Block/Neutralize, ICC/IF, IHC, WB
Recommend Dilution	Western Blot: 1:1000, Block/Neutralize, Immunocytochemistry/ Immunofluorescence: 1:2000, Immunohistochemistry: 1:500-1:1000, Immunohistochemistry-Paraffin: 1:500-1:1000
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to an internal portion of rat MCP1 (within residues 15-40). [Swiss-Prot# P14844]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	16 kDa
Gene Name	C-C motif chemokine ligand 2
Database Link	NP_002973 Entrez Gene 24770 RatEntrez Gene 6347 Human UniProt ID P13500
Background	MCP-1 (monocyte chemoattracctant protein-1, also called C-C motif chemokine 2 or CCL2) is a secreted protein belonging to intercrine beta (chemokine CC) family and it specifically act as a chemotactic factor to attracts monocytes, CD4+ T cells, NK cells as well as for CCR2 expressing cells, but not neutrophils. Both hematopoietic and non-hematopoietic cells that include monocytes/macrophages, dendritic cells, astrocytes, endothelial cells and fibroblasts express MCP-1, and monocytes/macrophages are the major source of MCP-1 in peripheral blood. Besides, expression at acute inflammation sites, MCP-1 is upregulated in chronic inflammation, including autoimmune disease, atherosclerosis, cancer, and chronic infection. MCP-1 has been implicated in both pro-inflammatory and anti-inflammatory responses, and is a potential intervention point for the treatment of various inflammatory/autoimmune diseases (multiple sclerosis, atherosclerosis, insulin-resistant diabetes, rheumatoid arthritis and lupus nephritis), cancers (breast and prostate cancer) and HIV-1/AIDS.
Synonyms	GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
Note	This MCP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~16 kDa. In ICC/IP secretory vesicles staining was observed in HeLa cells. IHC-Fr and B/N applications are reported in research publications with PMIDs 22402584 and 22778093 respectively.
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway

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