p95 NBS1 (NBN) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 0.5 ug/ml, Immunohistochemistry: 1:100, Immunohistochemistry-Paraffin: 1:100
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide made to an internal portion of the mouse NBS1 protein (within residues 150-200). [Swiss-Prot# Q9R207]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	97 kDa
Gene Name	nibrin
Database Link	NP_002476 Entrez Gene 27354 MouseEntrez Gene 4683 Human UniProt ID O60934
Background	NBS1 (Nijmegen breakage syndrome protein 1) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. NBS1's FHA domain binds phospho-Thr residues in Ser-X-Thr motifs present in DNA damage proteins, including Mdc1 and Ctp1, whereas, BRCT domains of NBS1 bind Ser-X-Thr motifs when Ser residue is phosphorylated. These phospho-dependent interactions are important for recruiting repair and checkpoint proteins to DSB sites and NBS1 is responsible for MRN complex's nuclear translocation. NBS1 itself does not possess enzymatic activity and contributes to DSB repair primarily by mediating protein-protein interactions at DNA breakage sites. The central region of NBS1 1 possesses several SQ motifs that are phosphorylated by ATM kinase via DNA damage response. NBS1's C-terminus contains a domain that interacts with ATM and recruits it to DSBs, and induces apoptosis in response to damage. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis.
Synonyms	AT-V1; AT-V2; ATV; NBS; NBS1; P95
Note	This NBS1 antibody is useful for Immunohistochemistry on paraffin embedded sections and Western blot, where a band is seen at ~97 kDa.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Homologous recombination

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