FTCD Mouse Monoclonal Antibody [Clone ID: 58K-9]

Specifications

Product Data
Clone Name	58K-9
Applications	ICC/IF, IHC, WB
Recommend Dilution	Western Blot: 1:2000 - 1:5000, Immunocytochemistry/ Immunofluorescence: 1:50 - 1:250, Immunohistochemistry: 1:100 - 1:200, Immunohistochemistry-Paraffin: 1:100 - 1:200, Immunohistochemistry-Frozen
Reactivity	Human, Mouse, Rat, Bovine, Canine, Chicken
Host	Mouse
Clonality	Monoclonal
Immunogen	58K Golgi Protein purified from rat liver [UniProt# O88618]
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ascites
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	58 kDa
Gene Name	formimidoyltransferase cyclodeaminase
Database Link	NP_996848 Entrez Gene 14317 MouseEntrez Gene 89833 RatEntrez Gene 10841 Human UniProt ID O95954
Background	The 58K Golgi protein is one of the most commonly used Golgi markers and has been proposed to implicate in linking Golgi to microtubules and it peripherally associates with cytosolic surface of Golgi membranes. This protein shows extensive overall sequence identity with porcine FTCD and has been suggested to represent rat FTCD (formiminotransferase-cyclodeaminase), a metabolic enzyme involved in the conversion of histidine to glutamic acid. FTCD's formiminotransferase activity facilitate transfer of a a formimino group from N-formimino-L-glutamic acid to tetrahydrofolate to generate glutamic acid and 5-formiminotetrahydrofolate, and the cyclodeaminase activity then converts the 5-formiminotetrahydrofolate to 5,10-methenyl tetrahydrofolate and ammonia. FTCD appears to be a dynamic component of Golgi and cycles between Golgi and earlier compartments of the secretory pathway. It also binds and promotes bundling of vimentin filaments originating from the Golgi, and mutations in this gene encoding for 58K Golgi Protein i.e. FTCD are associated with glutamate formiminotransferase deficiency (FIGLU-URIA).
Synonyms	LCHC1
Note	This 58K Golgi Protein antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry paraffin-embedded sections. In Western blot a band is observed at ~58 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24505439)
Reference Data
Protein Pathways	Histidine metabolism, Metabolic pathways, One carbon pool by folate

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