Calreticulin (CALR) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:20-1:100, Immunohistochemistry-Frozen, Immunohistochemistry: 1:20, Immunohistochemistry-Paraffin: 1:20, Western Blot: 1:1000-1:10000, Simple Western: 1:500, Knockdown Validated: 1:2500, Knockout Validated
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A full-length protein to mouse Calreticulin [UniProt# P14211]
Formulation	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	55 kDa
Gene Name	calreticulin
Database Link	NP_004334 Entrez Gene 12317 MouseEntrez Gene 811 Human UniProt ID P27797
Background	Calreticulin (CRT) is a ubiquitous multifunctional calcium-binding protein which attaches to the surface of cells and implicates in signal transduction events associated with innate immunity, cell adhesion, and apoptosis. Because CRT lacks a trans-membrane domain, CD91 (LDLR-related protein 1) has been proposed as a candidate anchoring receptor for this protein. CRT is mainly localized in ER lumen, cytoplasmic granules as well as in sarcoplasmic reticulum lumen as a monomer where it forms a component of an EIF2 complex (CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5) and interacts with GABARAP, NR3C1, PDIA3/ERp57, TRIM21 etc. CRT is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in ER via the calreticulin/calnexin cycle. It interacts with DNA-binding domain of NR3C1 for facilitating its nuclear export. CRT is involved in maternal gene expression regulation and participates in oocyte maturation via regulation of calcium homeostasis. In immune system, cell surface CRT (csCRT) serves as the signal-transducing receptor for collectin family proteins which binds to foreign organisms or apoptotic cells through their globular heads, while their collagen-like tails bind to csCRT, which leads to phagocytosis. CRT located in the cytoplasm, at the cell membrane and extracellular matrix functions in many physiological processes, such as cell adhesion, phagocytosis, and tumor recognition.
Synonyms	cC1qR; CRT; HEL-S-99n; RO; SSA
Note	This Calreticulin antibody is useful for Western blot, where a band at ~55 kDa is seen. Note that Calreticulin has several reported isoforms that range from 48-62 kDa. ICC data from customer review.
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transcription Factors
Protein Pathways	Antigen processing and presentation

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.