NUMB Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, Simple Western, WB
Recommend Dilution	Simple Western: 1:200, Immunocytochemistry/ Immunofluorescence: 1:500, Western Blot: 0.2-0.5 ug/ml
Reactivity	Human, Mouse, Chicken
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal region of mouse NUMB (between residues 600-653). [UniProt# Q9QZS3]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	NUMB, endocytic adaptor protein
Database Link	NP_003735 Entrez Gene 18222 MouseEntrez Gene 8650 Human UniProt ID P49757
Synonyms	C14orf41; c14_5527; S171
Note	This NUMB antibody is useful for Western blot and Immunocytochemistry/Immunofluorescence. In WB one may see any or all of the four isoforms. The molecular weight of human and mouse NUMB is 72 kDa (isoform 1), 66 kDa (isoform 2), 71 kDa (isoform 3), and 65 kDa (isoform 4). Multiple non-specific bands may be seen with lower dilutions and/or longer exposure times than suggested in the protocol we used to obtain our data.
Reference Data
Protein Pathways	Notch signaling pathway

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