SCARB1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Block/Neutralize, FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen (Negative): 5 ug/ml, Knockout Validated, Flow Cytometry: 1:400, Block/Neutralize, Immunohistochemistry: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:50-1:1000, Immunoprecipitation, Western Blot: 1:1000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII that was expressed as two tandem copies in bacteria using the pET system.
Formulation	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	82 kDa
Gene Name	scavenger receptor class B member 1
Database Link	NP_005496 Entrez Gene 20778 MouseEntrez Gene 25073 RatEntrez Gene 949 Human UniProt ID Q8WTV0
Background	SR-BI (scavenger receptor class BI, SCARB1) is a member of CD36 receptor family and act as a major receptor for HDL for facilitating selective lipid uptake from lipoproteins in several tissues. SR-BI gene gives rise to at least two mRNA splice variants (SR-BI and SR-BII), and interestingly, SR-BII variant is the main isoform in terms of mRNA levels in certain tissues e.g. SR-BII mRNA is 48-fold higher than SR-BI in brain cells; and protein levels reaching 10-15% of SR-BI in the liver. Similar differential SR-BII protein expression levels have also been detected in considerable amounts in other tissues, such as testes, retinal pigment epithelial cells, and spleen. SR-BII isoform differs from SR-BI only in its entirely different, yet highly conserved, cytoplasmic C-terminus which has been proposed to contains a signal responsible for marked alterations in cellular distribution as well as cellular trafficking of SR-BII compared with SR-BI. In contrast to SR-BI, SR-BII mediates the rapid uptake of significant amounts of HDL into endosomal recycling compartment by a pathway which is different from selective lipid uptake at the cell surface (mediated by SR-BI). Because SR-BII influence cellular cholesterol trafficking/homeostasis in a manner distinct from SR-BI, the relative expression as well as functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.
Synonyms	CD36L1; CLA-1; CLA1; HDLQTL6; SR-BI; SRB1
Note	This SR-BI/SR-BII antibody is useful for Flow Cytometry (PMID: 22097902), functional studies (customer feedback), Immunocytochemistry, Western blot and Immunohistochemistry. In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI and/or SR-BII such as liver, ovary, adrenal glands, and to as lesser extent testes, heart and mammary glands.
Reference Data
Protein Families	Druggable Genome, Transmembrane

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