LIMPII (SCARB2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Flow Cytometry, Immunocytochemistry/ Immunofluorescence: 1:25-1:100, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:25, Immunohistochemistry: 1:25
Reactivity	Human, Mouse, Rat, Bovine, Chinese Hamster, Hamste
Host	Rabbit
Clonality	Polyclonal
Immunogen	A peptide containing residues from mouse SR-BII (between residues 400-478) plus an N-terminal cysteine was coupled to KLH. [UniProt# O35114]
Formulation	Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	82 kDa
Gene Name	scavenger receptor class B member 2
Database Link	NP_005497 Entrez Gene 12492 MouseEntrez Gene 117106 RatEntrez Gene 950 Human UniProt ID Q14108
Background	Scavenger receptor class BI or SCARB1 gene belongs to CD36 receptor family and encodes for at least two mRNA splice variants which are SR-BI and SR-BII, among which SR-BII variant is the main isoform in terms of mRNA levels in certain tissues. For example, SR-BII mRNA is 48-fold higher than SR-BI in brain cells and protein levels reaching 10-15% of SR-BI in the liver. Similar differential SR-BII protein expression levels have also been detected in considerable amounts in other tissues, such as testes, retinal pigment epithelial cells, and spleen. SR-BII isoform differs from SR-BI only in its entirely different, yet highly conserved, cytoplasmic C-terminus which has been proposed to contains a signal responsible for marked alterations in cellular distribution as well as cellular trafficking of SR-BII compared with SR-BI. In contrast to SR-BI, SR-BII mediates the rapid uptake of significant amounts of HDL into endosomal recycling compartment by a pathway which is different from selective lipid uptake at the cell surface (mediated by SR-BI). Because SR-BII influence cellular cholesterol trafficking/homeostasis in a manner distinct from SR-BI, the relative expression as well as functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.
Synonyms	AMRF; CD36L2; EPM4; HLGP85; LGP85; LIMP-2; LIMPII; SR-BII
Note	This SR-BII antibody is useful for Immunohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at ~82 kDa in tissues that express SR-BII such as liver, testes, and adrenal glands. Flow Cytometry was reported in scientific literature.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Lysosome

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